Part I. Nuclear Transfer. Origin and Progress of Nuclear Transfer In Nonmammalian Animals. A. Culture and Maintenance of Nuclear Transfer Embryos. In Vitro Maturation and Embryo Production in Cattle. Activation of Oocytes After Nuclear Transfer. Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos. Maintenance of Pregnancy in Pigs With Limited Viable Embryos. Health and Neonatal Care of Bovine Clones. B. Nuclear Transfer Methods. Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice. Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors. A Double Nuclear Transfer Technique for Cloning Pigs. Nuclear Transfer in Nonhuman Primates. Cloning of Exotic/Endangered Species: Desert Bighorn Sheep. Handmade Somatic Cell Cloning in Cattle. C. Transgenesis. Genetic Modification of Sheep by

Methods in Molecular Biology™ • 348 NUCLEAR TRANSFER PROTOCOLS: CELL REPROGRAMMING AND TRANSGENESIS ISBN: 1-58829-280-0 E-ISBN: 1-59745-154-1 ISSN: 1064-3745

Nuclear Transfer With Gene-Targeted Somatic Cells. Transgenesis Using Nuclear Transfer in Goats. Part II. Reprogramming Nuclei. Nuclear Reprogramming: An Overview. Expression of Imprinted Genes in Cloned Mice. Nuclear Remodeling Assay in XenopusEgg Extract. In Vitro Reprogramming of Nuclei and Cells. Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells. Gene Expression and Methylation Patterns in Cloned Embryos. Part III. Concluding Remarks. Are There Any Normal Clones? Future and Applications of Cloning. Index.

ISBN l-Sflfl5T-5flD-D

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