Steve Pells

Department of Gene Function and Development Roslin Institute Roslin, Midlothian, UK

Humana Press ^ Totowa, New Jersey

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Cover design by Patricia F. Cleary

Cover illustrations: (Foreground) Reprogramming of DNA methylation during mouse preimplantation development: from the two-cell stage onwards, there is passive loss of DNA methylation attributable to exclusion of the maintenance DNA methyltransferase from the nucleus; de novo methylation is evident in the inner cell mass at the blastocyst stage. The upper row shows 5-Methylcytosine staining, and the lower row shows DNA staining in the same embryo (Fig. 1, Chap. 11; see complete figure and caption on p. 130 and discussion on pp. 130-131). (Background) Piezo-assisted injection of a cumulus cell nucleus into an MII oocyte (Fig. 3, Chap. 10; see complete caption and discussion on pp. 122-123).

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Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1

eISBN 1-59745-005-7 ISSN 1064-3745

Library of Congress Cataloging-in-Publication Data

Nuclear reprogramming : methods and protocols / edited by Steve Pells. p. ; cm. -- (Methods in molecular biology ; 325) Includes bibliographical references and index.ISBN 1-58829-379-3 (alk. paper)

1. Cell nuclei--Transplantation--Laboratory methods.[DNLM: 1. Cell Nucleus--physiology. 2. Cell Fusion--methods. 3. Transplantation--meth-ods. QU 350 N965 2006] I. Pells, Steve. II. Series: Methods in molecular biology (Clifton, N.J.) ; v. 325. QH442.2.N84 2006 660.6'5--dc22

2005020464

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