In Vivo Crosslinking and Chromatin Preparation

1. Phosphate-buffered saline (PBS): 140 mM NaC1, 2.5 mM KC1, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.5.

2. 37% Formaldehyde solution (Merck).

3. Quenching solution: 2 M glycine (Sigma) in PBS.

4. Lysis buffer: 10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 5 mM MgCl2, 0.2% NP40.

5. Glycerol buffer: 10 mM Tris-HCl, pH 7.4, 0.1 mM ethylenediaminetetraacetic acid (EDTA), 5 mM MgAc2, and 25% glycerol.

6. 2X MNase buffer: 50 mM KCl, 8 mM MgCl2, 2 mM CaCl2, and 100 mM Tris-HCl, pH 7.4, stored at room temperature.

7. Immunoprecipitation (IP) buffer: 25 mM Tris-HC1, pH 8.0, 2 mM EDTA, 150 mM NaC1, 1% Triton X100, 0.1% sodium dodecyl sulfate (SDS).

8. Protease inhibitor cocktail (Sigma), stored in aliquots at -20°C.

9. 50 mM Phenylmethyl sulfonyl fluoride (PMSF) in isopropanol, stored at room temperature.

12. EDTA 500 mM, pH 8.0, stored at room temperature.

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Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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