Media Reagents and Stock Solutions for Cell Culture

Phosphate-buffered saline (PBS) containing 10 g of NaCl, 0.25 g of KCl, 1.44 g of Na2HPO4, and 0.25 g of KH2PO42H2O L, filter-sterilized through a 0.22- im filter. 2. Trypsin solution 0.2 trypsin 1 250 (Serva, Heidelberg, Germany) in PBS for routine passaging, 0.1 trypsin 1 500 in PBS for replating of EB outgrowths, filter-sterilized through a 0.22- im filter. 3. Ethylenediamine tetraacetic acid (EDTA) solution 0.02 EDTA (Sigma, St. Louis, MO) in PBS for routine passaging, 0.08 EDTA in PBS for...