The Production And Biological Effects Of Radiodetoxified

In our laboratory E. coli bacteria are produced in bulk by fermentation and extracted by the phenol-water method of Westphal with some modifications [14]. The LPS is further purified by ultracentrifugation and dissolved in water by 60Co-gamma radiation using 50, 100, 150 or 200 kGy doses. The toxicity of LPS is decreased as shown in Fig. 1.

The biological effects of radiation-treated LPS are presented in Table I. The harmful effects of LPS are decreased after radiation, whereas its capacity to induce tolerance, to function as an immunoadjuvant and immunomodulator, to protect against shock and radiation and to stimulate natural resistance are preserved to a large extent. The lethal effect of such preparations (LD50

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