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Western blot, huntington expression in transfected cells, 280-282 limb-girdle muscular dystrophy proteins, analysis, 363 antibodies, 356, 357, 372 antibody staining, 363 apparatus, 357 blotting, 362 detection, 363 electrophoresis, 362 gel casting, 361 multiplex blot, 364

pre-staining of blot, 362, 363 principles, 359, 360 sample preparation, 360, 361 solutions and buffers, 357, 358, 372 staining and destaining of gel, 362 polyglutamate detection using antibody 1C2 affinity of antibody, 84 applications, 83, 84 blotting, 86-88 disease discovery, 84, 85 electrophoresis, 85, 87 gel casting, 85-88 immunodetection, 86-88 materials, 85, 86 protein extraction, 85, 86, 88 prion protein, diagnosis of disease, 306 electrophoresis, blotting, and detection, 309 materials, 308, 311 sample preparation, 309, 311

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