Reverse Transcription

1. Random hexamer primers, 100 yM. Store at -20°C.

2. RNase inhibitor (RNAguard, Amersham Pharmacia Biotech, Bucks, UK). Store at -20°C.

3. Superscript II reverse transcriptase, 200 U/yL (provided with 5X first strand buffer and 0.1 M DTT,) (Life Technologies Ltd., Paisley, Scotland). Store at -20°C.

4. dNTP mix: 1.25 mM each dNTP (Amersham Pharmacia Biotech). Store at -20°C.

5. Ribonuclease H, 1 U/yL (Life Technologies Ltd.). Store at -20°C.

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