EBV Transformation of B Lymphocytes

1. RPMI 1640 media with L-glutamin (Seromed Biochrom, Berlin, Germany).

2. Fetal calf serum (FCS) (Seromed Biochrom) (inactivate at 56°C for 30 min before use).

3. Penicillin/Streptomycin (10.000 IE/10,000 yg/mL) (Seromed Biochrom).

4. Cyclosporin A 100 yg/yL.

5. Epstein Barr virus EB95-8.

6. Biocoll 1.077 g/mL (Seromed Biochrom).

7. Puromycin dihydrochloride (Sigma, St. Louis, MO, USA) 50 mg/mL in sterile water.

8. Phosphate-buffered saline (PBS) (Seromed Biochrom).

9. Nunclon TC tubes flat (Nunc).

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