Myeloperoxidase and Sudan Black B

An essential cytochemical stain is one that demonstrates the presence of MPO, an enzyme restricted to the primary granules of granulocytes and monocytes.8 This enzyme can be identified by performing a benzidine-based peroxidase reaction or SBB staining or by diaminobenzidine reaction (DAB). The benzidine method is the best available method against which other methods should be compared. However, because of the concern for its potential carcinogenicity, it should not be adopted as the universal reference method.64

The SBB reaction, a general stain for intracellular lipids, can be used in the same way. This stain is present in both primary and secondary granules. Sudanophilia and MPO activity are closely parallel.65

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