Myeloid

such molecularly defined leukemia subtypes, since immuno-phenotyping can be performed more rapidly, at lower cost, and at more clinical leukemia laboratories as compared with molecular analyses.

When establishing new leukemia subclasses on the basis of antigen expression patterns alone, one should strive to unravel clinical relevance. Major efforts have been made to formulate a minimal panel of antibodies that will suffice to provide an informative immunophenotype and allow for standardized diagnostic interpretations. In fact, three antibody combinations can reliably discern the lineage trait (T versus B lymphoid versus myeloid) of a blast cell population (see below). If the goal is to describe novel leukemia subtypes or to discover prognostic markers, however, much more elaborate antibody panels must be tested.

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