Immunohistochemistry

Although flow cytometry is the method of choice for immunophenotyping, immunohistological examination of bone marrow sections has been proposed as an alternative method.68 Its advantages include identification of morphological features, the possibility of retrospective analysis, and in the situation where peripheral blood or marrow smears are inadequate for study such as bone marrow fibrosis or hypocellular acute leukemia. Paraffin reactive antibodies (i.e., CD34, MPO, CD68, antihemoglobin, and factor VIII-related antigen) can successfully be used to subclassify AML.6

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