Assays for Antibodies to Glycolipid Antigens

The solid-phase assay has also been adapted for glycolipid antigens (Smolarsky, 1980). The glycolipids are dissolved in ethanol and added to the wells of polyvinyl plates. The ethanol is then evaporated by a stream of nitrogen followed by high vacuum for 5 min, and the plates washed three times in PBS containing 0.3% gelatin and 1 mM ethylenediamine tetra-acetic acid (EDTA). The remainder of the assay is similar to those previously described. Other assays for antibodies to glycolipids have been described by Gray (1979), Young et al. (1979), and Handman and Jarvis (1985).

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