Fragmentation of Monoclonal Antibodies

Antibody Fragmentation Ficin

In certain circumstances, it may be desirable to generate antigen-binding fragments of monoclonal antibodies. For example, when cells with receptors for the Fc portion of IgG are present (e.g. macrophages, monocytes), intact IgG molecules may bind nonspecifically via their Fc portions (see Section 5.3.4). Removal of the Fc will prevent this mode of binding. The production of small fragments of IgM (intact Mr 900000) may aid penetration into the tissues for cytochemical studies. The older...