Figure 6.2. (a) Schematic diagram of the SBE reaction. (b) Typical example of a multiplexed SBE reaction detected by electrophoresis. Twenty autosomal SNPs were typed based on the nucleotide lengths of the SBE primers and the fluorescent label of the added dideoxynucleotide. The tested individual was heterozygous in four positions (SNP numbers 9, 10, 12 and 17) and homozygous in 16 positions can be analysed simultaneously by electrophoresis (Sanchez et al., 2005). In the electropherogram, the length of the SBE primer identifies the SNP locus and the colour of the fluorescent label identifies the SNP allele (Figure 6.2b).
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