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Quantification of Alu sequence targets by singleplex PCR

MtDNA quantification by singleplex PCR

Autosomal DNA and mtDNA quantification by duplex PCR

Autosomal DNA, Y chromosome and mtDNA quantification by triplex PCR

Alu Sequence

(124 bp) Inter-Alu sequences (pool of different sizes) Intra-Alu sequences (200-226 bp) HV1 (113 bp) HV1 (287 bp)

RB1 (78 bp) mtDNA coding region (142 bp) HUMTH01

(170-190 bp) MtDNA region ND1 (69 bp)

Nuclear Alu sequence (71 bp) Y-Chr target (69 bp) mtDNA target (79 bp)

SYBR Green

SYBR Green

SYBR Green

VIC-Taqman-MGB probe FAM-Taqman-MGB probe

FAM-TaqMan probe VIC-TaqMan probe

FAM-TaqMan probe VIC-Taqman-MGB probe (NED-TaqMan probe for IPC detection)

VIC-Taqman-MGB probe NED-Taqman-MGB probe FAM-Taqman-MGB probe

DNA degradation assessment by simultaneous quantification of two different sized fragments

Duplex PCR amplification for simultaneous quantification of nuDNA and mtDNA Duplex PCR amplification for simultaneous quantification of nuDNA and mtDNA Includes an internal standard to evaluate Taq inhibition Triplex PCR amplification for simultaneous quantification of nuDNA, mtDNA and male Y-DNA

(Andréasson et al., 2002, 2006) (Timken et al., 2005)

(Walker et al. 2005)

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