O N

I_I Addition of Phenol

Vortex + Centrifuge r 1 f 1 Transfer upper aqueous \7 V7 phase to a new tube

Addition of Phenol/ LJ Chloroform/Isoamyl V Alcohol 25:24:1

Vortex + Centrifuge

Transfer upper aqueous phase to a new tube r 1 Addition of Chloroform/ v-7 Isoamyl Alcohol 24:1

Vortex + Centrifuge r 1 r 1 Transfer upper aqueous \7 V7 phase to a new tube

P^-l Addition of 2x LJ volumes Ethanol

Centrifuge + Wash the Pellet Resuspension of DNA in Water b: CHELEX

Lysis

Addition of Water

Initial Incubation at RoomTemperature

Vortex + Centrifuge

Remove Supernatant

Addition of 5% CHELEX ® 100 + Proteinase K

Vortex + Centrifuge

Addition of 5% CHELEX ® 100 + Proteinase K

Incubation (37 or 56°C)

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