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96 swabs in 2.2 ml well plates

0 ml

User can choose between aqueous + swab sample; blood samples were preprocessed, swabs + card punches were centrifuged; no detectable cross-contamination Manual addition of reagents; bar-code possible; contamination in resuspended DNA; technical problems, require significant further development; poor PCR success rate; presence of protein0 Manual addition of reagents; bar-code possible; problems with vacuum, require further development; one of best results; satisfactory DNA yield + purity0

Autogen NA-3000EUa

Promega MagneSila

Integrated system

BiomekĀ® 2000 (3000 with new heater, shaker + deep-well plates0-9)

DNA IQTM

Paramagnetic beads

Salt extraction

Paramagnetic particles

Manual addition of reagents; bar-code possible; problems not chemistry specific; needs extensive redesign; DNA yield quite poor, but good quality0 Simple to operate; addition of reagents + sample tracking must be performed manually, therefore risk of cross-contamination; no integrated bar-code system; 8% extraction failure rate0 User can choose between aqueous + swab sample; blood samples were preprocessed, swabs + card punches were centrifuged; no detectable cross-contamination

0 Evaluation report of automated extraction methodologies of the National Genetics Reference Laboratory (NGRL, Wessex, UK: http://www.

ngrl.co.uk/Wessex/extraction.htm).

b Anslinger et ol. (2005) and Montpetit et ol. (2005).

c Forensic applications are known.

d Nagy et ol. (2005) and Steinlechner et ol. (2005).

9 Greenspoon et ol. (2004), Crouse et ol. (2005) and McLaren et ol. (2006).

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