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a For abbreviated protein names, see the legend to Table 13.1.

b ELISA results are an indicator of the affinity and the titre of the antibody in sera samples obtained in our studies. The values are determined by diluting the sera samples until ELISA staining reaches the background level.

a For abbreviated protein names, see the legend to Table 13.1.

b ELISA results are an indicator of the affinity and the titre of the antibody in sera samples obtained in our studies. The values are determined by diluting the sera samples until ELISA staining reaches the background level.

unteers were transferred onto filter paper discs, dried and stored under different environmental conditions. Following a 9-month storage period under the conditions outlined in Table 13.3, paper discs were incubated with trypsin/Tween-20 for 24 hours at 37°C to achieve solubilization and digestion of the samples. Inactivation of trypsin by heating was followed by the addition of protease inhibitors. The samples were used in the competitive displacement assay in which either labelled synthetic peptides or labelled pooled human tryptically digested plasma were used. It was possible to match some of the five samples in most cases tested, except for the samples from two batches, most probably due to complete loss of the proteinaceous matter. The sensitivity of detection can be improved by reducing the reaction volume (in the above experiment, 5 |l of whole blood was used per 1-ml assay). Table 13.4 presents some of the examples in which all five different simulated forensic samples were matched correctly (with or without false positives). Pearson's correlation coefficient was calculated for each pair of arrays (identically made arrays, assayed with differently stored samples) and was used as an indicator of matching (or mismatching)

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