The Southern Blot

The DNA blot was developed by Southern in 1975 (3), and it remains the method of choice among many researchers for

From: Molecular Diagnostics: For the Clinical Laboratorian, Second Edition Edited by: W. B. Coleman and G. J. Tsongalis © Humana Press Inc., Totowa, NJ

reliable, quantitative detection of specific DNA sequences. The Southern blot can detect the presence of homologous genes across species. For example, if a biologically relevant gene has been located in the rat, it is possible to construct a labeled probe from the rat gene and use it to search for a homologous gene in the human genome by Southern blot analysis. The DNA blot can also be used to assess the relative copy number of a specific gene. This particular application of the Southern blot is useful in detecting gene amplification, which is a common response to environmental pressure and often accounts for drug resistance in mammalian cells (4,5). Southern analysis can identify mutations, deletions, or rearrangements that alter the integrity of a specific gene, which can be useful in the prognosis of certain types of cancer and in the prenatal diagnosis of genetic diseases. In addition, the DNA blot is a valuable tool for molecular cloning, providing a mechanism for localization of specific sequences to defined fragments within larger bacteriophage and cosmid genomic DNA clones.

Pregnancy Guide

Pregnancy Guide

A Beginner's Guide to Healthy Pregnancy. If you suspect, or know, that you are pregnant, we ho pe you have already visited your doctor. Presuming that you have confirmed your suspicions and that this is your first child, or that you wish to take better care of yourself d uring pregnancy than you did during your other pregnancies; you have come to the right place.

Get My Free Ebook


Post a comment