The Northern Blot

The Northern blot allows identification of specific messenger RNA sequences within a mixture of RNA molecules. The final signal achieved on the blot is proportional to the number of specific sequences present, allowing for a quantitative analysis of gene expression. The RNA transcripts produced from a particular gene can vary in size because of phenomena such as the following:

1. Utilization of a secondary transcription start site by RNA polymerase;

2. Premature termination of transcription resulting from nonsense mutation;

3. Posttranscriptional modifications, such as splicing;

4. Deletions within the gene coding sequence.

These alternative transcripts can be detected by Northern blot analysis. This information is routinely used to determine if the expression of a specific gene is altered in any way. Aberrations in gene expression are frequently studied in the laboratory to evaluate the cellular response to a particular stimulus or treatment at the molecular biology level. Because abnormal expression of specific genes is often reliably associated with certain disease states, the Northern blot can also be a valuable tool for diagnosis/prognosis in the clinical setting.

Pregnancy Guide

Pregnancy Guide

A Beginner's Guide to Healthy Pregnancy. If you suspect, or know, that you are pregnant, we ho pe you have already visited your doctor. Presuming that you have confirmed your suspicions and that this is your first child, or that you wish to take better care of yourself d uring pregnancy than you did during your other pregnancies; you have come to the right place.

Get My Free Ebook


Post a comment