S

Lane 1:

RhCcEe 1200bp RhD 600l>p

Rli.0 mbp KJiCVEe 129bp

Fig. 1. RHD genotyping: (A,B) Illustrations of targeted exons and introns of RHCE, RHD, and RHDy for RHD and RHD^genotyping, respectively (14,18,23,27). (C) RHD genotyping by ASPCR and 2% agarose gel electrophoresis. Products of each analysis are run in pairs; the intron 4/exon 10 standard RHD reaction is in the first lane of each pair and the RHD ^reaction is in the second lane of each pair. The RHD amplification generates 600-bp and 193-bp RHD-specific products, as well as 1200-bp and 129-bp internal control products derived from RHCE. The RHD^reaction generates a 381-bp product from a normal RHD allele and a 418-bp product from RHDy alleles, a primer pair targeting RhCE provides an internal control (14,18,23,27). Set A: normal RhD-negative individual; set B: normal RHD-positive individual; set C: normal RHD-positive/RHDy heterozygous individual; panel D/panel E: prenatal genotyping case involving a serologically RhD-negative/standard genotype-positive mother possessing the RHDy allele (panel D), and the initially ambiguous fetal genotyping (panel E). Lane S: 100-bp DNA ladder (Gibco/BRL, Gaithersburg, MD).

Fig. 1. RHD genotyping: (A,B) Illustrations of targeted exons and introns of RHCE, RHD, and RHDy for RHD and RHD^genotyping, respectively (14,18,23,27). (C) RHD genotyping by ASPCR and 2% agarose gel electrophoresis. Products of each analysis are run in pairs; the intron 4/exon 10 standard RHD reaction is in the first lane of each pair and the RHD ^reaction is in the second lane of each pair. The RHD amplification generates 600-bp and 193-bp RHD-specific products, as well as 1200-bp and 129-bp internal control products derived from RHCE. The RHD^reaction generates a 381-bp product from a normal RHD allele and a 418-bp product from RHDy alleles, a primer pair targeting RhCE provides an internal control (14,18,23,27). Set A: normal RhD-negative individual; set B: normal RHD-positive individual; set C: normal RHD-positive/RHDy heterozygous individual; panel D/panel E: prenatal genotyping case involving a serologically RhD-negative/standard genotype-positive mother possessing the RHDy allele (panel D), and the initially ambiguous fetal genotyping (panel E). Lane S: 100-bp DNA ladder (Gibco/BRL, Gaithersburg, MD).

Pregnancy Guide

Pregnancy Guide

A Beginner's Guide to Healthy Pregnancy. If you suspect, or know, that you are pregnant, we ho pe you have already visited your doctor. Presuming that you have confirmed your suspicions and that this is your first child, or that you wish to take better care of yourself d uring pregnancy than you did during your other pregnancies; you have come to the right place.

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