1. Southern, E. M. Blotting at 25. TIBS 25:585-588, 2000.

2. Porchet, N. and Aubert, J.-P. Northern blot analysis of large mRNAs, in Glycoprotein Methods and Protocols: The Mucins, Methods in Molecular Biology Vol. 125, Corfield , A., ed., Humana, Totowa, NJ.

3. Southern, E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517, 1975.

4. Ruiz, J. C., Choi, K. H., von Hoff, D. D., Roninson, I. B., and Wahl, G. M. Autonomously replicating episomes contain mdr1 genes in a multidrug-resistant human cell Line. Mol. Cell. Biol. 9:109-115, 1989.

5. Stark, G. R. and Wahl, G. M. Gene amplification. Annu. Rev. Biochem. 53:447-491, 1984.

6. Sambrook, J. F., Fritsch, E. F., and Maniatis, T., eds., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Press, Cold Spring Harbor, NY, 1992.

7. Kroczek, R. A. Southern and northern analysis. J. Chromatogr. 618:133-145, 1993.

8. Bostian, K. A., Lee, R. C., and Halvorson, H. O. Preparative frac-tionation of nucleic acids by agarose gel electrophoresis. Anal. Biochem. 95:174-182, 1979.

9. Knowland, J. S. Polyacrylamide gel electrophoresis of nucleic acids synthesised during the early development of Xenopus laevis Daudin. Biochim. Biophys. Acta 204:416-429, 1970.

10. Preat, T. High resolution southern analysis of genomic DNA using heat denatured acrylamide gels. Nucleic Acids Res. 18:1073, 1990.

11. Vesterburg, O. A. A short history of electrophoretic methods. Electrophoresis 14:1243-1249, 1993(abstract).

12. Burmeister, M. and Ulanovsky, L. Pulsed-Field Gel Electrophoresis, Humana, Totowa, NJ, 1992.

13. Van Oss, C. J., Good, R. J., and Chaudhury, M. K. Mechanism of DNA (Southern) and protein (Western) blotting on cellulose nitrate and other membranes. J. Chromatogr. 391:53-65, 1987.

14. Wahl, G. M., Stern, M. and Stark, G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc. Natl. Acad. Sci. USA 76:3683-3687, 1979.

15. Chomczynski, P. One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal. Biochem. 201:134-139, 1992.

16. Ming, Y. Z., Di, X., Gomez-Sanchez, E. P., and Gomez-Sanchez, C. E. Improved downward capillary transfer for blotting of DNA and RNA. Biotechniques 16:58-59, 1994.

17. Smith, M. R., Devine, C. S., Cohn, S. M., and Lieberman, M. W. Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose. Anal. Biochem. 137:120-124, 1984.

18. Kroczek, R. A. and Siebert, E. Optimization of northern analysis by vacuum-blotting, RNA-transfer visualization, and ultraviolet fixation. Anal. Biochem. 184:90-95, 1990.

19. Stacey, J. and Isaac, P. G. Restriction enzyme digestion, gel electrophoresis, and vacuum blotting of DNA to nylon membranes. Methods Mol. Biol. 28:25-36, 1994.

20. Olszewska, E. and Jones, K. Vacuum blotting enhances nucleic acid transfer. Trends Genet. 4:92-94, 1988.

21. Khandjian, E. W. UV crosslinking of RNA to nylon membrane enhances hybridization signals. Mol. Biol. Rep. 11:107-115, 1986.

22. Kornguth, S. E., Anderson, J. W., Scott, G., and Kubinski, H. Fractionation of subcellular elements from rat central nervous tissue in a cesium chloride gradient. Biochemical and ultrastructural studies. Exp. Cell Res. 45:656-670, 1967.

23. Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159, 1987.

24. Lehrach, H., Diamond, D., Wozney, J. M., and Boedtker, H. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743-4751, 1977.

25. McMaster, G. K. and Carmichael, G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc. Natl. Acad. Sci. USA 74:4835-4838, 1977.

26. Kroczek, R. A. Immediate visualization of blotted RNA in northern analysis. Nucleic Acid. Res. 17:9497, 1989.

27. Wade, M. F. and O'Conner, J. L. Using a cationic carbocyanine dye to assess RNA loading in Northern gel analysis. Biotechniques 12:794-796, 1992.

28. Hagel, L., Lundstrom, H., Andersson, T., and Lindblom, H. Properties, in theory and practice, of novel gel filtration media for standard liquid chromatography. J. Chromatogr. 476:329-344, 1989.

29. Kaguni, J. M. and Kaguni, L. S. Enzyme-labeled probes for nucleic acid hybridization. Methods Biochem. Anal. 36:115-127, 1992.

30. Butler, E. T. and Chamberlin, M. J. Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme. J. Biol. Chem. 257:5772-5778, 1982.

31. Yamaguchi, K., Zhang, D., and Byrn, R. A. A modified nonradioactive method for northern blot analysis. Anal. Biochem. 218: 343-346, 1994.

32. Dubitsky, A., Brown, J., and Brandwein, H. Chemiluminescent detection of DNA on nylon membranes. Biotechniques 13:392-400, 1992.

33. Murakami, A., Tada, J., Yamagata, K., and Takano, J. Highly sensitive detection of DNA using enzyme-linked DNA-probe. 1. Colorimetric and fluorometric detection. Nucleic Acids Res. 17:5587-5595, 1989.

34. Nakagami, S., Matsunaga, H., Oka, N., and Yamane, A. Preparation of enzyme-conjugated DNA probe and application to the universal probe system. Anal. Biochem. 198:75-79, 1991.

35. Schaap, A. P., Akhavan, H., and Romano, L. J. Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes. Clin. Chem. 35:1863-1864, 1989.

36. Ingram, V. M. A specific chemical difference between the globins of normal human and sickle-cell anemia hemoglobin. Nature 178:792-794, 1956(abstract).

37. Chang, J. C. and Kan, Y. W. A sensitive new prenatal test for sickle-cell anemia. N. Engl. J. Med. 307:30-32, 1982.

38. Burkitt, D. and O'Connor, G. T. Malignant lymphoma in African children. A clinical syndrome. Cancer 14:258-269(abstract).

39. Scriver, C. R., Beaudet,A. L., Sly, W. S., and Valle, D., eds. The Metabolic Basis of Inherited Disease, 6th ed., McGraw-Hill, New York, 1989.

40. Fearon, E. R. and Vogelstein, B. A genetic model for colorectal tumorigenesis. Cell 61:759-767, 1990.

Pregnancy Guide

Pregnancy Guide

A Beginner's Guide to Healthy Pregnancy. If you suspect, or know, that you are pregnant, we ho pe you have already visited your doctor. Presuming that you have confirmed your suspicions and that this is your first child, or that you wish to take better care of yourself d uring pregnancy than you did during your other pregnancies; you have come to the right place.

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