234 56789

Fig. 4. Fragment-size analysis using slab gels and autoradiography: Fragile X. The autoradiograph shows PCR products using primers flanking the CCG repeat 5' of the FMR1 gene. Sizes of patient alleles are determined in reference to the A lanes from a sequencing reaction using phage M13 as the template. The arrows indicate the position of the 204 and 254 base fragments of the sequencing ladder. Lanes 1, 4, and 6 show hemizygous males with amplicons of 200 bases, or 29 repeats. Lanes 2, 5, and 7 are from males with 30 repeats, Lane 3 shows a male with 43 repeats. Lane 8 shows a female heterozygous for a premutation allele of 52 repeats and a normal allele of 31 repeats. Lane 9 shows a control female sample with 30 and 87 repeats. Lane 10 is a no-DNA amplification control. (Courtesy of the Mayo Clinic Molecular Genetics Laboratory.)

Pregnancy Guide

Pregnancy Guide

A Beginner's Guide to Healthy Pregnancy. If you suspect, or know, that you are pregnant, we ho pe you have already visited your doctor. Presuming that you have confirmed your suspicions and that this is your first child, or that you wish to take better care of yourself d uring pregnancy than you did during your other pregnancies; you have come to the right place.

Get My Free Ebook

Post a comment