Fig. 4. Fragment-size analysis using slab gels and autoradiography: Fragile X. The autoradiograph shows PCR products using primers flanking the CCG repeat 5' of the FMR1 gene. Sizes of patient alleles are determined in reference to the A lanes from a sequencing reaction using phage M13 as the template. The arrows indicate the position of the 204 and 254 base fragments of the sequencing ladder. Lanes 1, 4, and 6 show hemizygous males with amplicons of 200 bases, or 29 repeats. Lanes 2, 5, and 7 are from males with 30 repeats, Lane 3 shows a male with 43 repeats. Lane 8 shows a female heterozygous for a premutation allele of 52 repeats and a normal allele of 31 repeats. Lane 9 shows a control female sample with 30 and 87 repeats. Lane 10 is a no-DNA amplification control. (Courtesy of the Mayo Clinic Molecular Genetics Laboratory.)
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