Fish Vs Other Techniques For Cytogenetic Analysis

For the obvious reasons like high sensitivity, technical ease, applicability to a wide range of clinical specimens, and the feasibility of combining with phenotypic characterization, FISH has been welcomed in routine clinical diagnostic practices. FISH, while taking advantage of the foundation of cytopatho-logical and histopathological competency, provides an additional spectacle revealing genomic secrets of the cellular pathology. This matrimony of cell and molecular biology is thus a major advantage offered by FISH.

The classical cytogenetic analysis with metaphase chromosomal banding continues to be a gold standard in genetic testing laboratories. However, several emerging studies report beyond the shadow of doubt that certain chromosomal abnormalities show a tendency not to grow well in vitro and/or result in poor spreading of chromosomes in metaphase preparations. Monosomy 7 in AML and MDS, t(15;17) in AML and +12 and 13q-/—13 in CLL were thus found to be frequently underestimated by conventional G-banding, and interphase-FISH provided the necessary sensitivity for their accurate estimation (78). The underestimation of t(15;17) and trisomy 12 cells by G-banding has been thought to result from their slow prolifer-ative rates, leading to being outgrown in culture (79,80). On the other hand, a good correlation between G-banding and FISH

Table 6

A Comparative View of FISH, PCR, and RT-PCR

Table 6

A Comparative View of FISH, PCR, and RT-PCR

Characteristic

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