Brain slices

Brain slices have played a pivotal role in developing theories on the operation of neuronal networks. Slices about 0.4 mm thick are cut from the brains of deeply anaesthetised or recently killed experimental animals, or sometimes from humans undergoing neurosurgery. Brain slices can survive many hours in vitro in an artificial cerebrospinal fluid, usually equilibrated with 95 per cent oxygen and 5 per cent carbon dioxide (with bicarbonate providing a pH buffer). If the slices are prepared under sterile conditions, they can survive for weeks as 'organotypic slice cultures'. In both cases the visualization of the anatomy of the living slice helps locate electrodes, the mechanical stability greatly simplifies recordings from inside neurones, and the lack of a blood-brain barrier facilitates drug applications and changes in ion concentrations. Brain slices have proved immensely popular and successful, but it is important to remember that they are only one tool in the armoury needed to study brain function, and that ultimately results from them must be put in the context of the whole organism.

Invisible Viagara

Invisible Viagara

You are about to discover the "little-known" techniques, tricks and "mind tools" that will show you how to easily "program" your body and mind to produce an instant, rock-hard erection. Learn how to enjoy all of the control, confidence and satisfaction that comes from knowing you can always "rise to the challenge" ... and never have to deal with embarrassment, apologies, shyness or performance anxiety in the bedroom, ever again.

Get My Free Ebook

Post a comment