Identification Methods

Any of the previous methods will be supported by extensive databases from the manufacturers, which will include the results of probably thousands of determinations for the selected genera. It is unlikely that any such extensive cross-comparison could be performed by the user.

However, the methods may be based on completely different principles, e.g., biochemical metabolism, DNA probes, GC analysis of fatty acids etc., to methods currently being used. Hence the identification provided may differ at the species level and possibly at the genus level.

For most of these methods validation is limited to the following factors:

• Ruggedness

• Robustness

The normal microorganisms identified in a particular application should be considered. For example, if a manufacturer is constantly identifying Gram-negative nonfermenting bacteria, then more organisms representing this category should be included in the validation.

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