In this technique the culture is grown in the presence of a radiolabeled protein for a short period, and is then incorporated into the cells. A suspension of the culture is applied to an electrophoresis plate, and a high voltage applied to separate the cell protein into a band, which can then be visualized by exposure to x-ray film. This banding is unique to each species of microorganism.
The authors are unaware of any commercial application of the method, which also requires specialized techniques and equipment. The reader is referred to Proteomics Review 2001 by Michael Durin for further information
Was this article helpful?