Pulsedfield gel electrophoresis

Pulsed-field gel electrophoresis (Schwartz and Cantor, 1984) is commonly used to distinguish structural variation among microbial strains on a genome-wide basis (Tenover et al., 1995). Structural variants are represented by chromosomes or restriction fragments with altered electrophoretic mobilities; thus PFGE identifies the genomic regions that harbor variation, but does not provide any further evidence as to the molecular nature or sequence information involved with each event, except through toilsome Southern blot analysis.

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