Info

454 array pyrosequencing

100 x

Not possible

$250

<40?

SAM + 454 pyrosequencing

100 x

Possible?

$250

>90?

bAdditional cost for mate pair and large clone handling and sequencing.

bAdditional cost for mate pair and large clone handling and sequencing.

oversampling than is used for conventional GS20 pyrosequencing or equivalent short-read technology. Our coverage is some 10-fold higher than used for conventional Sanger sequencing. However, the cost differences between array pyrosequencing and Sanger sequencing are approximately 100-120-fold per megabasepair of Phred20 sequence data, to the favour of GS20 array pyrosequencing.

We suggest that the integration of SAM and SBS technologies will advance de novo array-based sequencing, dramatically reducing sequencing cover needed for larger genomes to a maximum defines by SAM theory, while improving sequencing accuracy, the depth of cover and the length of sequence that can be reassembled correctly from SBS read data. Here, we chose to illustrate the effect of SAM mutation of DNA targets by in silico simulation of completely random mutations at any base in the target region to a level of 10%. We have previously described Bayesian methods that use the proportion of observed mutations to weight the predicted reassembled sequence (Keith et al., 2003). We would also suggest that Bayesian mathematical methods could contribute to more efficient assembly of short-read SAM sequence data.

0 0

Post a comment