Artem E Men3

1 Capitalbio Corporation: National Engineering Research Centre for Beijing Biochip Technology, 18 Life Science Parkway, Changping District, Beijing 102206, China 2Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing

100084, China

3AGRF, Institute of Molecular Bioscience, University of Queensland, St. Lucia, Queensland

4072, Australia

4AGRF, Walter and Eliza Hall Institute for Medical Research, 1G Royal Parade, Parkville,

Victoria 3050, Australia

Contents

1. Introduction 4 1.1. Biotechnological implications of ultra-high-throughput sequencing capability 6

2. Advanced sequencing technologies 9

2.1. Capillary electrophoresis and Sanger sequencing 9

2.2. High-throughput capillary-array sequencing 9

2.3. Signal detection dyes and detectors 10

2.4. Microchip electrophoresis 11

2.5. Capillary electrophoretic sequencing on microcapillary chips 11

2.6. Sequencing by mass spectrometry 14

3. Solid-phase array sequencing devices 15

3.1. Ultra-sensitive detectors and sequencers 15

3.2. Sequencing by synthesis 15

3.3. Single DNA molecule sequencing 19

3.4. Hybridization re-sequencing 22

4. Future technologies 22

4.1. Nanopore membranes 23

4.2. Direct electrical detection of DNA synthesis 25

5. Applied short-read genomic sequencing 25

5.1. Genotyping by re-sequencing 25

5.1.1. Polony genotyping 26

5.1.2. Pyrosequencer genotyping 26

5.1.3. Polymorphism ratio sequencing 26

5.1.4. BEAMing 27

5.2. PaleoGenomics 27

5.3. Neanderthal genomics 28

5.4. MetaGenomics 29

PERSPECTIVES IN BIOANALYSIS, VOLUME 2 ISSN 1871-0069 DOI: 10.1016/S1871-0069(06)02001-5

© 2007 Elsevier B.V. All rights reserved

5.5. SAM sequencing of repetitive DNAs

5.6. Transcriptome and expressed RNA sequence analysis

5.7. MPSS and genome analysis

5.8. Optical mapping

0 0

Post a comment