1. A clean catch sample of urine is obtained from the patient.

2. Sample is spun down in a cytocentrifuge to obtain a cytospin preparation of urothelial cells.

3. Staining and rinsing with Perl's reagent (Prussian blue) is performed on the glass slides.

4. Examine under oil-immersion lens of microscope.

5. Haemosiderin stains as blue dots within urothelial cells.

6. Ignore all excess stain, staining outside cells or in debris all of which are common.

7. True positive is only when clear detection within urothelial squames is seen.

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