In suspected case at delivery cord blood is tested for

• Presence of antibody on fetal RBCs by DAT.

• Hb and blood film (spherocytes, increased polychromasia, NRBCs).

Maternal blood is tested for

• Serum antibodies against fetal cells (by indirect antiglobulin test, IAGT).

• Kleihauer test—detects and quantitates fetal RBCs in maternal circulation.

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