Esr632

Haematinic assays 633

Haemoglobin electrophoresis 633

Haptoglobin 633

Schumm's test 633

Kleihauer test 633

Reticulocytes 634

Urinary haemosiderin 635

Ham's test 636

Immunophenotyping 636

Cytogenetics 638

HLA typing 640

Rapid analysis by the latest generation automated blood counters using either forward angle light scatter or impedance analysis provides enumeration of leucocytes, erythrocytes and platelets and quantification of haemoglobin, MCV plus derived values for haematocrit, MCH and MCHC, red cell distribution width (a measure of cell size scatter), mean platelet volume and platelet distribution width and a 5 parameter differential leucocyte count. The counter also flags samples which require direct morphological assessment by examination of a blood film.

Sample: peripheral blood EDTA; the sample should be analysed in the laboratory within 4h.

Blood film

Morphological assessment of red cells, leucocytes and platelets should be performed by an experienced individual of all samples in which the FBC has revealed any result significantly outside the normal range, samples in which a flag has been indicated by the automated counter and if clinically indicated. A manual differential leucocyte count may be performed and may differ from that produced by the automated counter most notably in patients with haematological disease affecting the leucocytes.

Sample:peripheral blood EDTA; the sample should be analysed in the laboratory within 4h. May be made directly from drop of blood or EDTA sample, air-dried and fixed.

Plasma viscosity

This test is a sensitive but non-specific index of plasma protein changes which result from inflammation or tissue damage. The plasma viscosity is unchanged by haematocrit variations and delay in analysis up to 24h and is therefore more reliable than the ESR. It is not affected by sex but is affected by age, exercise and pregnancy.

632 Sample: peripheral blood EDTA; the sample should be analysed in the laboratory within 24h.

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