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IgC anti-Hsp70 autoantibodies are present in the normal immunoglobulin preparation (IVIG) and in normal human serum (NHS). ELISA plates were coated with human heat shock protein 70(2 ig ml) and incubated with serial dilutions of IVIG (A) or NHS(B). Uncoated wells were used as control. Anti-Hsp70 IgG antibodies were determined as described earlier.23 Means of two parallel measurements and SD are plotted, the experiment was repeated four times with identical results. anti-Hsp60...

Mechanisms Underlying Phenotypic Plasticity in Inducible Cold Tolerance

Murray, Andrew Y. Gracey and Andrew R. Cossins* The physiological adjustment of organisms in response to temperature variation is a crucial part of coping with environmental stress. An important component of the cold response is the increase in membrane lipid unsaturation, and this has been linked to an enhanced resistance to the debilitating or lethal effects of cold. Underpinning the lipid response is the upregulation of fatty acid desaturases (des),...

C elegans Models for Neurodegenerative Disease and Aging

Morley and Richard I. Morimoto* Abstract A growing number of human neurodegenerative diseases are associated with the expression of misfolded proteins that oligomerize and form aggregate structures. Over time, accumulation of misfolded proteins leads to the disruption of cellular protein folding homeostasis and eventually to cellular dysfunction and death. To investigate the relationship between misfolded proteins, neuropathology and aging, we have developed models...

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Work with the genetic manipulation of A9-desaturase in C. elegans offers the first direct test in an animal species of the relationship between lipid saturation and the cold tolerance phe-notype and our results indicate that, at best, the lipid response accounts for only a small fraction of acquired cold tolerance. C. elegans in many ways is an ideal organism to study the mechanisms underpinning environmental adaptation, with access to RNAi libraries and mutant strains etc., providing the...

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Cellular fate organization protein fate transcription metabolism Figure 3. Hsp90 influences multiple cellular processes. The yeast Hsp90 interactors identified by Zhao et al.20 are distributed among a variety of functional categories. Functional categories which contain more than 10 of interactors for each screening method are listed. 2H screens for yeast Hsp90 have also been carried out by Millson et al.27'28 Initially, the group used full length Hsc82 as bait however, even with DBD domain...