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Ulrich Vogel

Institut für Hygiene und Mikrobiologie Universität Würzburg Josef-Schneider-Str. 2 97080 Würzburg Germany

Wilma Ziebuhr

Institut für Molekulare Infektionsbiologie Röntgenring 11 97070 Würzburg Germany

Neil Stoker

The Royal Veterinary College Pathology and Infectious Diseases Royal College Street London, NW1 0TU United Kingdom

Color Plates

Fig. 3.2 Protein pattern of heat-shocked B. subtilis cells. During exposure to heat shock (37-to48°C), B. subtilis cells were pulse-labeled with 35S-l-methionine. After the separation of crude protein extract by 2-D gels, the proteins were visualized by silver staining and the protein synthesis rate was

Fig. 3.2 Protein pattern of heat-shocked B. subtilis cells. During exposure to heat shock (37-to48°C), B. subtilis cells were pulse-labeled with 35S-l-methionine. After the separation of crude protein extract by 2-D gels, the proteins were visualized by silver staining and the protein synthesis rate was determined by phosphoimaging. The two images were overlaid with the aid of the Delta-2D software package (dual-channel imaging technique [11]; Decodon, Greifswald, Germany). The red-labeled proteins form the heat stress stimulon. (This figure also appears on page 48.)

Fig. 3.4 The phosphate starvation stimulon of B. subtilis consists of both general stress proteins also induced by phosphate starvation (rB regulon, rB-dependent proteins marked in blue) and starvation-specific proteins (PhoR regulon, marked in red). The stress/starvation induction profile of both groups and the functions ofthe proteins

Fig. 3.4 The phosphate starvation stimulon of B. subtilis consists of both general stress proteins also induced by phosphate starvation (rB regulon, rB-dependent proteins marked in blue) and starvation-specific proteins (PhoR regulon, marked in red). The stress/starvation induction profile of both groups and the functions ofthe proteins are indicated. C, control; h, heat stress; e, ethanol stress; s, NaCl stress; a, pH 5.5; o, oxidative stress (H2O2); p, puromycin; g, glucose starvation; O2, oxygen starvation; ph, phosphate starvation; aa, amino acids starvation. (This figure also appears on page 50.)

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