RNA Extraction

Rapid RNA extraction is a vital step in a microarray experiment if it aims to assess gene expression levels in a meaningful manner. The RNA sample tested must reflect transcription levels as they were naturally and not as a result of culture manipulation or RNA degradation. The first step is therefore usually immediate immersion of the culture in a "stop solution" such as guanidinium thiocyanate that halts all biological activity including transcription and enzyme-mediated degradation of RNA. The cell must then be lysed and the contents disrupted, the exact method depending on the bacterial species being used. It is quite common now for commercial kits to be used for the RNA extraction, but essentially the RNA is purified by precipitation and resuspended in RNase-free water. Many laboratories have their own favored method of extracting nucleotide samples from bacterial cultures, and the protocols will vary depending on the species under study.

Biological Replicates

RNA Reference Culture Experimental Sample

RNA Reference Culture Experimental Sample

Technical Replicates

Dye Swap Multiple Arrays

RNA Reference Culture Experimental Sample

RNA Reference Culture Experimental Sample

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