1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.

2 Sander, C. 2000. Genomic medicine and the future of health care. Science 287:1977-1978.

3 Hensel, M., and D. W. Holden. 1996. Molecular genetic approaches for the study of virulence in both pathogenic bacteria and fungi. Microbiology 142:1049-1058.

4 Reyrat, J.-M., F. X. Berthet, and B. Gic-quel. 1995. The urease locus ofMycobac-terium tuberculosis and its utilization for the demonstration of allelic exchange in Mycobacterium bovis bacillus Calmette-Guerin. Proc Natl Acad Sci USA 92:8768-8772.

5 Mahairas, G. G., P. J. Sabo, M. J. Hickey, D. C. Singh, and C. K. Stover.

1996. Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis. J. Bacteriol. 178:1274-1282.

6 Tinsley, C. R., andX. Nassif. 1996. Analysis of the genetic differences between Neisseria meningitidis and Neisseria gonorrhoeae: two closely related bacteria expressing two different pathogenici-ties. Proc Natl Acad Sci USA 93:1110911114.

7 Hood, D. W., M. E. Deadman, M. P. Jennings, M. Bisercic, R. D. Fleischmann, J. C. Venter, and E. R. Moxon. 1996. DNA repeats identify novel virulence genes in Haemophilus influenzae. Proc Natl Acad Sci USA 93:11121-11125.

8 Coutte, L., R. Antoine, H. Drobecq, C. Locht, and F. Jacob-Dubuisson. 2001. Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway. EMBO J 20:5040-5048.

9 Fields, P. I., R. V. Swanson, C. G. Hai-daris, and F. Heffron. 1986. Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent. Proc Natl Acad Sci USA 83:51895193.

10 Fields, P. I., E. A. Groisman, and F. Heffron. 1989. A Salmonella locus that controls resistance to microbicidal proteins from phagocytic cells. Science 243:1059-1062.

M. D. Jones, E. Dalton, and D. W. Holden. 1995. Simultaneous identification of bacterial virulence genes by negative selection. Science 269:400-403.

12 Mecsas, J. 2002. Use of signature-tagged mutagenesis in pathogenesis studies. Curr Opin Microbiol 5:33-37.

A. Camilli. 2002. Identification of novel factors involved in colonization and acid tolerance of Vibrio cholerae. Mol Microbiol 43:1471-1491.

14 Cox, J. S., B. Chen, M. McNeill, and W. R. Jacobs Jr. 1999. Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 402:79-83.

15 Sassetti, C. M., D. H. Boyd, and E. J. Rubin. 2001. Comprehensive identification of conditionally essential genes in mycobacteria. Proc Natl Acad SciUSA 98:12712-12717.

16 Sassetti, C. M., and E. J. Rubin. 2003. Genetic requirements for mycobacterial survival during infection. Proc Natl Acad Sci USA 100:12989-12994.

17 Coelho, P. S. R., A. Kumar, and M. Snyder. 2000. Genome-wide mutant collections: toolboxes for functional geno-mics. Curr Opin Microbiol 3:309-315.

18 Ross-Macdonald, P., P. S. Coelho, T. Roemer, S. Agarwal, A. Kumar,

R. Jansen, K. H. Cheung, A. Sheehan, D. Symoniatis, L. Umansky, et al. 1999. Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature 402:413-418.

J. D. Boeke, H. Bussey, et al. 1999. Functional characterization of the S. cerevi-siae genome by gene deletion and parallel analysis. Science 285:901-906.

20 Scherens, B., and A. Goffeau. 2004. The uses of genome-wide yeast mutant collections. Genome Biol 5:229.

21 Lum, P. Y., C. D. Armour, S. B. Stepa-niants, G. Cavet, M. K. Wolf, J. S. Butler, J. C. Hinshaw, P. Garnier, G. D. Pre-stwich, A. Leonardson, et al. 2004. Dis covering modes of action for therapeutic compounds using a genome-wide screen of yeast heterozygotes. Cell 116:121-137.

22 Hughes, T. R., M. D. Robinson, N. Mit-sakakis, and M. Johnston. 2004. The promise of functional genomics: completing the encyclopedia of a cell. Curr Opin Microbiol 7:546-554.

23 Kobayashi, K., S. D. Ehrlich, A. Alber-tini, G. Amati, K. K. Andersen, M. Arnaud, K. Asai, S. Ashikaga, S. Aymer-ich, P. Bessieres, et al. 2003. Essential Bacillus subtilis genes. Proc Natl Acad Sci USA 100:4678-4683.

Y. Qiu, D. Frisch, K. M. Winterberg, and F. R. Blattner. 2004. Systematic mutagenesis of the Escherichia coli genome. J Bacteriol 186:4921-4930.

25 Datsenko, K. A., and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97:6640-6645.

26 Hutchison, C. A., S. N. Peterson, S. R. Gill, R. T. Cline, O. White, C. M. Fraser, H. O. Smith, and J. C. Venter. 1999. Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286:2165-2169.

27 Jacobs, M. A., A. Alwood, I. Thaipisutti-kul, D. Spencer, E. Haugen, S. Ernst, O. Will, R. Kaul, C. Raymond, R. Levy, et al. 2003. Comprehensive transposon mutant library of Pseudomonas aerugi-nosa. Proc Natl Acad Sci USA 100:14339-14344.

E. M. Glass, F. Aslund, O. Schneewind, and D. M. Missiakas. 2004. Staphylococ-cus aureus virulence genes identified by bursa aurealis mutagenesis and nema-tode killing. Proc Natl Acad Sci USA 101:12312-12317.

29 Geoffroy, M. C., S. Floquet, A. Metais, X. Nassif, and V. Pelicic. 2003. Large-scale analysis of the meningococcus genome by gene disruption: resistance to complement-mediated lysis. Genome Res 13:391-398.

30 Pelicic, V., S. Morelle, D. Lampe, and X. Nassif. 2000. Mutagenesis of Neisseria meningitidis by in vitro transposi tion of Himarl mariner. J Bacteriol 182:5391-5398.

31 Carbonnelle, E., S. Helaine, L. Prouven-sier, X. Nassif,and V. Pelicic. 2005. Type IV pilus biogenesis in Neissseria meningitidis: PilW is involved in a step occurring after pilus assembly, essential for fiber stability and function. Mol Microbiol 55:54-64.

32 Helaine, S., E. Carbonnelle, L. Prouven-sier, J. L. Beretti, X. Nassif, and

V. Pelicic. 2005. PilX, a pilus-associated protein essential for bacterial aggregation, is a key to pilus-facilitated attachment of Neisseria meningitidis to human cells. Mol Microbiol 55:65-77.

33 Alm, R. A., A. J. Bodero, P. D. Free, and J. S. Mattick. 1996. Identification of a novel gene, pilZ, essential for type 4 fimbrial biogenesis in Pseudomonas aeruginosa. J Bacteriol 178:46-53.

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