Fig. 3.5 Multicolor imaging of expression patterns under different growth conditions in B. subtilis. Delta-2D software (Decodon) was used to visualize complex protein expression patterns on the 2-D gel image in the standard pH range 4-7. The color code is presented in the top left corner. Proteins only induced by single stresses are colored red (H, heat), light-blue (O, oxidative stress), and orange

(E, ethanol), respectively Proteins induced by oxidative stress as well as heat stress are colored yellow, proteins induced by ethanol stress and heat stress are colored dark-blue, proteins induced by oxidative and ethanol stress can be recognized as purple spots, and, finally, proteins induced by all three stimuli are displayed in green. (This figure also appears on page 51.)


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