Microarray FollowUp

Microarray data often depend on the conditions in which the bacteria were studied, the method of RNA isolation, the statistical analysis, and so on. As a result, there is a lot of uncertainty about using microarray data as concrete evidence. Because of this, it is common for researchers to use microarrays as an exploratory tool that generate leads which can be followed up individually with simpler, more developed and robust (but lower-throughput) processes such as RTq-PCR or Northern blots. RTq-PCR (quantitative or real-time PCR) is frequently used to validate at least some of the results from microarrays.

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