Genome Scale Mutational Analyses

The fundamental limitation of the above mutational approaches is that, no matter how large in scale they are, they can never be actually exhaustive since the collections of mutants are undefined and always incomplete. By identifying all the genes that constitute an organism, genome sequences provided the missing information for the construction of a comprehensive collection of mutants, which would be suitable for actually assessing gene function on a global scale. For this reason, although the creation of a comprehensive arrayed collection of mutants remains a daunting challenge, the potential of such collections for the identification of all the genes involved in a defined biological process and, ultimately, the exact contribution of each gene to microbial life [17] have prompted several pro jects for the construction of these toolboxes based on approaches initially developed in the model organism Saccharomyces cerevisiae.

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