Result Interpretation

In T-cell clonality assessment by PCR, bands may be difficult to interpret when they are weak or outside the expected size range. Weak results should be repeated to confirm reproducibility. Capillary electrophoresis instruments are useful for differentiating oligoclonal proliferations from clonal peaks due to their ability to separate PCR products at the single base pair level. Peaks or bands outside the expected diagnostic range could be definitively characterized by direct DNA sequencing. However, specific criteria for interpretation of PCR methods for clonality testing have not been established at this time.

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