Protein Truncation Test

The protein truncation test (PTT) is used to identify mutations that result in premature termination of protein translation. PTT detects mutations at the protein rather than the genomic DNA or RNA levels. Initially developed for Duchenne muscular dystrophy (DMD) testing, PTT now is used more widely,50 since protein-truncating mutations are associated with multiple types of hereditary cancer syndromes, including breast, ovarian, and colon.

For PTT, the gene segment of interest is amplified by PCR. The amplicons are used for in vitro transcription and translation in a coupled reaction. The resulting proteins are separated using sodium dodecyl sulfate (SDS) polyacry-lamide gel electrophoresis. The presence of a premature termination codon is indicated by the visualization of a lower-molecular-weight protein band than for the wildtype protein. Relatively large gene fragments (2-4kb) can be analyzed using PTT.

Examples of Applications of PTT

1. Duchenne muscular dystrophy diagnosis

2. BRCA1 and BRCA2 mutation detection51

3. APC mutations in colorectal cancer52

10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook


Post a comment