Poor Specimen Quality

The quality of extracted DNA or RNA is not usually a problem with fresh or frozen tissue or cells, if viable tissue has been obtained. However, poor DNA quality is a consistent problem with PCR assays of paraffin-embedded tissue, and this tissue is not suitable for RNA extraction and testing in most cases. Different fixatives will affect the success of DNA extraction; formalin or zinc-formalin fixation allows adequate DNA preservation for PCR in many cases, but it is most often not possible to obtain adequate DNA for PCR from most paraffin-embedded tissue fixed with mercury-based (B5), Zencker, or Bouin fixatives.

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