Problems Of Detection Of P450 In The Brain

As is evident from Table 1, several known forms of P450 can be detected in the brain by RT-PCR. Because of the extreme sensitivity of this method, it is not easy to evaluate the biological relevance of the mRNA detected by RT-PCR. This is not to say that transcripts expressed at low levels in the brain are of no physiological relevance. Because of the division of labor in brain cells, it is to be expected that some genes are expressed in a small specific population of cells and measurement of their expression in whole brain will produce a deceptively low value. In order for biological relevance to be considered, RT-PCR data must be accompanied by evidence that the protein is expressed in the brain and that it is localized in a limited number of cells rather than being present at trace levels in all cells.

Table 1

Xenobiotic, Steroid, and Fatty-Acid Metabolizing P450S in the Rat"

Table 1

Xenobiotic, Steroid, and Fatty-Acid Metabolizing P450S in the Rat"

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