Coded microbeads bound to oligonucleotides Illumina

Technology from Illumina (San Diego, CA) uses oligonucleotides linked to beads which are individually optically coded. These beads are then bound to the tips of individual fibers in a fiber-optic bundle (see figure 7.4). The company states that currently 2000 different genes can be represented on each fiber bundle, but appears to be scalable from 50,000 to 5 million beads per bundle. In addition, each array contains between 96 and 1536 bundles. Each bead can be optically interrogated by laser excitation via the fiber-optic bundle, using a proprietary technique. This technology is very much in the early testing stage and we will not have a good idea of its reproducibility and accuracy until after more independent testing.

Figure 7.4: The Illumina BeadArray.

If this technology does in fact prove viable, then its potential scalability to millions of probes will allow new ground to be reached in profiling. For example, millions of probes would allow each gene in the human genome to be represented, as well as all known polymorphisms and alternative splicing products.

Figure 7.4: The Illumina BeadArray.

If this technology does in fact prove viable, then its potential scalability to millions of probes will allow new ground to be reached in profiling. For example, millions of probes would allow each gene in the human genome to be represented, as well as all known polymorphisms and alternative splicing products.

Was this article helpful?

0 0

Post a comment