Techniques to Dissect Cellular and Subcellular Function in the Drosophila Nervous System

Heinrich J. G. Matthies and Kendal Broadie

Department of Biological Sciences Vanderbilt University Nashville, Tennessee 37235

I. Introduction

II. Background Sources of Information

III. Drosophila as a Genetic Model System for Molecular Neurobiology

A. Rapid Reverse Genetic Approaches for Initial Assessment of Gene Function

B. Generation of Mutations in a Gene of Interest

IV. Biochemistry of the Drosophila Nervous System

V. Cell Biology Techniques in the Drosophila Nervous System

A. The Drosophila Life Cycle

B. Protein Expression Studies with Immunocytochemistry

C. Transgenic Protein Expression Studies

D. Clonal Techniques for Mutant Analyses

VI. Functional Analysis Techniques in the Drosophila Nervous System

A. Imaging and Manipulating Calcium Levels in Drosophila Neurons

B. Monitoring Membrane Dynamics with Stryl Dyes

C. Monitoring Protein Dynamics in Living Drosophila Neurons VII. Drosophila Electrophysiology

A. Recording from Cultured Neurons

B. Recording from the Neuromuscular Junction

C. Recording from Central Motor Neurons

D. The Characterized Drosophila Circuit: Giant Fiber System

E. Recording from Sensory Systems

F. Future Directions: Recording from the Adult Brain References

METHODS IN CELL BIOLOGY, VOL. 71 Copyright 2003, Elsevier Science (USA). All rights reserved. 0091-679X/03 $35.00

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