Info

Insulin

10 mg/ml

in 0.01 M HCL

Tri-iodothyronine

200 ^g/ml

in 0.01 M NaOH

Progesterone

0.63 mg/ml

in EtOH absolute

Corticosterone

2 mg/ml

in EtOH absolute

^-Estradiol

100 ^g/ml

in EtOH absolute

B. Preparing 50 ml of 10 x N9 supplement from component stock solutions

To 40 ml of preferred medium (we use L15+), add the following volumes of the individual component stocks

B. Preparing 50 ml of 10 x N9 supplement from component stock solutions

To 40 ml of preferred medium (we use L15+), add the following volumes of the individual component stocks

Selenium dioxide

1 ml

Putrescine

1 ml

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