Info

Ingredient

Amount

Final concentration

Leibovitz's L-15 medium (GibcoBRL)

242 ml

96.8% (v/v)

D-(+)-Glucose solution (45%) (Sigma)

3.3 ml

0.6% (w/v)

200 mM L-glutamine (GibcoBRL)

2.5 ml

2 mM

Penicillin (10,000 units/ml) streptomycin

2.5 ml

100 units/ml

(10,000 ^g/ml) (Sigma)

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