Background Sources of Information

A number of volumes have been written about fly development, genetics, husbandry, and general molecular/cellular protocols (listed in Table I). The primary references are provided here: (1) "The Genome of Drosophila melanogaster,'' by Lindsley and Zinn; (2) ''Drosophila: A Laboratory Manual'' and ''Drosophila: A Laboratory Handbook,'' both by Ashburner; (3) ''Drosophila, A Practical Approach,'' edited by D. B. Roberts; (4) Fly Pushing: The Theory and Practice of Drosophila Genetics by R. J. Greenspan; (5) ''The Embryonic Development of Drosophila melanogaster,'' by J. A. Campos-Ortega and V. Hartenstein; (6) a two

Table I

List of Important Resources for Drosophila melanogaster

Books: Comprehensive information about Drosophila development, genetics, husbandry, and protocols "The Genome of Drosophila melanogaster" by L. Lindsley and G. G. Zimm (1992) ''Drosophila: A Laboratory Manual,'' edited by M. Ashburner (1989) ''Drosophila: A Laboratory Handbook,'' edited by M. Ashburner (1989) ''Drosophila, a Practical Approach,'' edited by D. B. Roberts, (1998)

''Fly Pushing: The Theory and Practice of Drosophila Genetics,'' by R. J. Greenspan (1997) ''The Embryonic Development of Drosophila melanogaster," by J. A. Campos-Ortega and V. Hartenstein (1998)

''The Development of Drosophila melanogaster," Vol. I and II, edited by M. Bate and A. Martinez Arias, (1993)

''Drosophila melanogaster: Practical Uses in Cell and Molecular Biology,'' edited by L. S. B. Goldstein and E. A. Fyrberg (1994)

''Drosophila Protocols,'' edited by W. Sullivan, M. Ashburner and R. S. Hawley (2000)

Specific chapters on Drosophila neurobiological techniques

In ''Drosophila Protocols,'' edited by W. Sullivan, M. Ashburner, and R. Scott Hawley, (2000). H. Bellen and V. Budnik: The Neuromuscular Junction

T. Wolff: Histological Techniques for the Drosophila Eye. Part I: Larva and Pupa T. Wolff: Histological Techniques for the Drosophila Eye. Part II: Adult K. S. Broadie: Electrophysiological Approaches to the Neuromusculature K. S. Broadie: Functional Assays of the Peripheral and Central Nervous Systems S. Sweeney, A. Hidalgo, J. S. deBelle, and H. Keshishian: Functional Cell Ablation J. Hirsch: Exposing Drosophila to Neuroactive Drugs In ''Drosophila: A Practical Approach,'' edited by D. B. Roberts (1998)

Connolly, J. G., and T. Tully: Behaviour, Learning, and Memory. In Drosophila melanogaster: Practical Uses in Cell and Molecular Biology,'' edited by L. S. B. Goldstein and E. A. Fyrberg (1994)

N. H. Patel: Imaging Neuronal Subsets and Other Cell Types in Whole-Mount Drosophila Embryos and Larvae Using Antibody Probes

Specific volumes and chapters on Drosophila developmental neurobiology ' 'Neuromuscular Junctions in Drosophila," edited by V. Budnik and L. S. Gramates (1999)

In ''The Development of Drosophila melanogaster," Vol. I, edited by M. Bate and A. Martinez Arias (1993)

M. Bate.: The Mesoderm and Its Derivatives

C. S. Goodman and C. Q. Doe: Embryonic Development of the Drosophila Central Nervous System Y. N. Jan and L. Y. Jan: The Peripheral Nervous System

Useful internet sites

http://flybase.bio.indiana.edu/

Extensive information about Drosophila biology and research. Information on maps, genes, proteins, alleles, stocks, vectors, and references; addresses for researchers in the fly community http://www.fruitfly.org/

Berkeley Drosophila Genome Project (BDGP). Information about the genomic projects at the center and how to obtain reagents, including ESTs, genomic clones, and multiple P-element collections. Also a site for Drosophila protocols http://edgp.ebi.ac.uk/

European Drosophila Genome Project (EDGP). Information about the genomic projects at the center and how to obtain reagents, including ESTs, genomic clones, and multiple P-element collections. Also a site for Drosophila protocols

(Continues)

Table I (Continued)

List of Important Resources for Drosophila melanogaster

http://flybrain.neurobio.arizona.edu/

Interactive site specializing in anatomy of the fly brain http://flybrain.uni-freiburg.de/Flybrain/html/atlas/index.html

Interactive site specializing in anatomy of the fly brain http://flybrain.nibb.ac.jp/

Interactive site specializing in anatomy of the fly brain http://www.fly-trap.org/

Searchable database for expression patterns of Ga14 lines http://www.its.caltech.edu/~ zinnlab/motoraxons/fma%20home%20page.html

Anatomy, development, and methods for Drosophila neuromuscular junction; Kai Zinn web page http://www.neuro.uoregon.edu/doelab/doelab.html

A description of neuroblast maps and lineages, including 3D and DiI labeling; Chris Doe web page http://ww.biochem.northwestern.edu/carthew/manual/Manual.html

Carthew laboratory protocols, including RNAi in Drosophila embryos; Richard Carthew web page volume set ''The Development of Drosophila melanogaster," edited by M. Bate and A. Martinez Arias; (7) ''Drosophila melanogaster: Practical Uses in Cell and Molecular Biology,'' edited by L. S. B. Goldstein and E. A. Fryberg; and (8) ''Drosophila Protocols,'' edited by W. Sullivan, M. Asburner, and R. S. Hawley. Several of these sources have chapters written specifically about Drosophila neuro-biological techniques, including (1) a chapter on immunocytochemistry of subsets of neurons (''Drosophila melanogaster: Practical Uses in Cell and Molecular Biology,'' edited by L. S. B. Goldstein and E. A. Fryberg); (2) a chapter on immunocytochemistry at the neuromuscular junction (H. J. Bellen); (3) two chapters on histological techniques for the eye (T. Wolff); (4) two chapters on electrophysiological techniques at the neuromuscular junction (NMJ) and the central nervous system (CNS) (K. Broadie); (5) a chapter on neuronal cell ablation (S. Sweeney et al.); and (6) a chapter on exposing Drosophila to neuroactive drugs (J. Hirsch). These latter five chapters are all in ''Drosophila Protocols,'' edited by W. Sullivan, M. Asburner, and R.S. Hawley. These chapters give extensive detail about many methods used by fly neurobiologists. A review of various aspects of the most commonly used neuronal system in flies, the neuromuscular junction, may be found in ''Neuromuscular Junctions in Drosophila,'' edited by V. Budnik and L. S. Gramates.

An online database about Drosophila can be found at http://flybase.bio.india-na.edu/, which contains extensive information about many aspects of Drosophila biology and research. Information about maps, genes, proteins, alleles, stocks, vectors, and references can be found at this site, as well as addresses for people in the fly community. Different information can be found at the Berkeley Drosophila Genome Project (BDGP, http://www.fruitfly.org/) and European Drosophila Genome Project (EDGP, http://edgp.ebi.ac.uk/). These sites contain information about the genomic projects at these centers and information about obtaining various reagents, including expressed sequence tags (ESTs), genomic clones, and multiple P-element collections. These sites also host an array of Drosophila protocols.

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