Diode array detection (DAD) with HPLC Nuclear magnetic resonance (NMR) Mass spectrometry (MS)

Circular dichroism

Related Observation

Used to study anthocyanins in living tissues, to elucidate the anthocyanin substitution pattern, among others After the HPLC separation, this methodology permits the online tentative identification of anthocyanin pigments The complete structural elucidation of a new anthocyanin structure requires the use of NMR of protons and carbons Evaluation of the molecular mass of the anthocyanin is important in the establishment of the anthocyanin structure Used to study the copigmentation phenomena

Source: Adapted from Delgado-Vargas et al. (2000).17

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